WB
Western blot analysis of Ionotropic glutamate receptor 2 + 3 on different lysates with Rabbit anti-Ionotropic glutamate receptor 2 + 3 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | Ionotropic glutamate receptor 2 + 3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human GRIA2 aa 834-883 / 883. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GRIA2 |
|---|---|
| Gene Synonyms | GLUR2 GLURB GluA2 HBGR2 NEDLIB gluR-2 gluR-B GluR-K2 |
| Gene Full Name | glutamate ionotropic receptor AMPA type subunit 2 |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | AMPA selective glutamate receptor 2 antibody AMPA selective glutamate receptor 3 antibody GluR K2 antibody GluR K3 antibody GLURB antibody GLURC antibody GLUTAMATE RECEPTOR 2 antibody GLUTAMATE RECEPTOR 3 antibody Glutamate receptor ionotropic AMPA 2 antibody Glutamate receptor ionotropic AMPA 3 antibody GRIA2 antibody GRIA3 antibody |
| Molecular Weight(MW) | 99 kDa |
| Cellular Localization | Cell membrane, Endoplasmic reticulum membrane, Postsynaptic cell membrane, Postsynaptic density membrane. |
WB
Western blot analysis of Ionotropic glutamate receptor 2 + 3 on different lysates with Rabbit anti-Ionotropic glutamate receptor 2 + 3 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
