WB
Western blot analysis of Desmoglein 2 on different lysates with Rabbit anti-Desmoglein 2 antibody at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.| Product Name | Desmoglein 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Desmoglein 2 aa 961-1,118 / 1,118. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DSG2 |
|---|---|
| Gene Synonyms | HDGC CDHF5 |
| Gene Full Name | desmoglein 2 |
| Gene Summary | This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jan 2016] |
| Alternative Names | ARVC 10 antibody ARVC10 antibody ARVD 10 antibody ARVD10 antibody Cadherin family member 5 antibody CDHF 5 antibody CDHF5 antibody CMD1BB antibody Desmoglein 2 antibody Desmoglein-2 antibody Desmoglein2 antibody DSG 2 antibody DSG2 antibody DSG2_HUMAN antibody HDGC antibody HDGC included antibody Human Desmoglein colon antibody MGC117034 antibody MGC117036 antibody MGC117037 antibody |
| Molecular Weight(MW) | 122 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of Desmoglein 2 on different lysates with Rabbit anti-Desmoglein 2 antibody at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
