WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BMAL1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Rat pancreas was stained with anti-BMAL1 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: BMAL1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | BMAL1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BMAL1 |
|---|---|
| Gene Synonyms | TIC JAP3 MOP3 ARNTL PASD3 ARNTL1 BMAL1c bHLHe5 |
| Gene Full Name | basic helix-loop-helix ARNT like 1 |
| Gene Summary | The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. The protein regulates interferon-stimulated gene expression and is an important factor in viral infection, including COVID-19. [provided by RefSeq, Oct 2021] |
| Alternative Names |
ARNTL BHLHE5 BMAL1 MOP3 PASD3 Aryl hydrocarbon receptor nuclear translocator-like protein 1 Basic-helix-loop-helix-PAS protein MOP3 Brain and muscle ARNT-like 1Class E basic helix-loop-helix protein 5 bHLHe5 Member of PAS protein 3 PAS domain-containing protein 3 bHLH-PAS protein JAP3 |
| Molecular Weight(MW) | 69 kDa(Observed band size: 78 kDa) |
| Cellular Localization | Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BMAL1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Rat pancreas was stained with anti-BMAL1 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: BMAL1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
