WB
Western blot analysis of EPHX2 on different lysates with Rabbit anti-EPHX2 antibody at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | EPHX2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human EPHX2 aa 1-50/555. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EPHX2 |
|---|---|
| Gene Synonyms | CEH SEH ABHD20 |
| Gene Full Name | epoxide hydrolase 2 |
| Gene Summary | This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] |
| Alternative Names | Bifunctional epoxide hydrolase 2 antibody CEH antibody Cytosolic epoxide hydrolase antibody EPHX2 antibody Epoxide hydratase antibody Epoxide hydrolase 2 antibody Epoxide hydrolase 2 cytoplasmic antibody epoxide hydrolase 2 cytosolic antibody Epoxide hydrolase soluble antibody HYES_HUMAN antibody SEH antibody Soluble epoxide hydrolase antibody |
| Molecular Weight(MW) | 63 kDa(Observed band size: 70 kDa) |
| Cellular Localization | Peroxisome, Cytoplasm. |
WB
Western blot analysis of EPHX2 on different lysates with Rabbit anti-EPHX2 antibody at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
