WB
Western blot analysis of Lipocalin-2 on different lysates with Rabbit anti-Lipocalin-2 antibody at 1/2,500 dilution. Exposure time: 3 minutes; Negative expression of Lipocalin-2 protein in mouse brain tissue is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Lipocalin-2 Recombinant Rabbit Monoclonal Antibody, 1/2,500 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Spleen. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Lipocalin-2 Recombinant Rabbit Monoclonal Antibody, 1/100, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Product Name | Lipocalin-2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2500 IHC:1:100 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LCN2 |
|---|---|
| Gene Synonyms | p25 24p3 MSFI NGAL |
| Gene Full Name | lipocalin 2 |
| Gene Summary | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015] |
| Alternative Names | 24p3 antibody 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody Alpha 2 microglobulin related protein antibody HGNC:6526 antibody HNL antibody Lcn 2 antibody Lcn2 antibody Lipocalin-2 antibody Migration stimulating factor inhibitor antibody MSFI antibody Neutrophil gelatinase associated lipocalin antibody Neutrophil gelatinase associated lipocalin precursor antibody Neutrophil gelatinase-associated lipocalin antibody NGAL antibody Oncogene 24p3 antibody Oncogenic lipocalin 24p3 antibody p25 antibody Siderocalin antibody siderocalin LCN2 antibody SV40 induced 24P3 protein antibody Uterocalin antibody |
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Secreted, Cytoplasmic granule lumen, Cytoplasmic vesicle lumen. |
WB
Western blot analysis of Lipocalin-2 on different lysates with Rabbit anti-Lipocalin-2 antibody at 1/2,500 dilution. Exposure time: 3 minutes; Negative expression of Lipocalin-2 protein in mouse brain tissue is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Lipocalin-2 Recombinant Rabbit Monoclonal Antibody, 1/2,500 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Spleen. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Lipocalin-2 Recombinant Rabbit Monoclonal Antibody, 1/100, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Application Notes | WB:1:2500 IHC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
