WB
Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000.IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Tubulin Antibody (green) was diluted at 1:200 (4℃ overnight). FoxO1 (Phospho Ser256) Rabbit pAb (red) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit AbFluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse AbFluor 488 was diluted at 1:1000 (room temperature, 50min).| Product Name | FoxO1 (Phospho Ser256) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272. |
| Modification | p-Ser256 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | DrosophilaHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO1 |
|---|---|
| Gene Synonyms | FKH1 FKHR FOXO1A |
| Gene Full Name | forkhead box O1 |
| Gene Summary | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
| Alternative Names |
FOXO1 FKHR FOXO1A Forkhead box protein O1 Forkhead box protein O1A Forkhead in rhabdomyosarcoma |
| Molecular Weight(MW) | 82 kDa |
| Cellular Localization | Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120 , PubMed:12228231 , PubMed:19221179 , PubMed:21245099 , PubMed:20543840 , PubMed:25009184) . In osteoblasts , colocalizes with ATF4 and RUNX2 in the nucleus (By similarity) . Serum deprivation increases localization to the nucleus , leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity) . Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads , via stimulation of Thr-24 phosphorylation , to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865 , PubMed:12228231) . Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation (PubMed:18356527) . Phosphorylation by NLK results in nuclear export (By similarity) . Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 (PubMed:19221179 , PubMed:21245099) . SGK1-mediated phosphorylation also results in nuclear translocation (By similarity) . Retained in the nucleus under stress stimuli including oxidative stress , nutrient deprivation or nitric oxide (By similarity) . Retained in the nucleus on methylation (By similarity) . PPIA/CYPA stimulates its nuclear accumulation (PubMed:31063815) . Deacetylation by SIRT6 , promotes its translocation into the cytoplasm (PubMed:25009184) . . |
WB
Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000.
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Tubulin Antibody (green) was diluted at 1:200 (4℃ overnight). FoxO1 (Phospho Ser256) Rabbit pAb (red) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit AbFluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse AbFluor 488 was diluted at 1:1000 (room temperature, 50min).| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
