IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Application: IHC-Fr. Species: Mouse. Site: cerebrum. Sample: Frozen section. Antibody concentration: 1/200. Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.IF-F
Application: IHC-Fr. Species: Rat. Site: cerebrum. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.| Product Name | Homeobox protein cut-like 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse Homeobox protein cut-like 1 aa 1,000-1,200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHC |
| IHC:1:500-1:1000 IF-F:1:200-1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CUX1 |
|---|---|
| Gene Synonyms | CDP CUX p75 CASP CDP1 COY1 Clox GDDI p100 p110 p200 CUTL1 GOLIM6 NEDDMS CDP/Cut Cux/CDP Nbla10317 |
| Gene Full Name | cut like homeobox 1 |
| Gene Summary | The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011] |
| Alternative Names | CCAAT displacement protein antibody CDP antibody CDP/Cut antibody CDP1 antibody Clox antibody COY1 antibody Cut homeobox antibody Cut homolog antibody Cut like 1 antibody Cut like homeobox 1 antibody CUTL1 antibody CUX antibody Cux/CDP antibody CUX1 antibody Golgi integral membrane protein 6 antibody GOLIM6 antibody Homeobox protein cux 1 antibody Nbla10317 antibody p100 antibody p110 antibody p200 antibody p75 antibody Putative protein product of Nbla10317 antibody |
| Molecular Weight(MW) | 166 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P53564 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-Homeobox protein cut-like 1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Application: IHC-Fr. Species: Mouse. Site: cerebrum. Sample: Frozen section. Antibody concentration: 1/200. Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.
IF-F
Application: IHC-Fr. Species: Rat. Site: cerebrum. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.| Application Notes | IHC:1:500-1:1000 IF-F:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
