WB
Western blot analysis of lysates from HeLa and 293 cells, using CARD10 Antibody. The lane on the right is blocked with the synthesized peptide.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4℃ overnight.3,Secondary antibody was diluted at 1:200 (room temperature, 45min).ICC/IF
Immunofluorescence analysis of HeLa cells, using CARD10 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | CARD 10 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CARD10. AA range:481-530. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CARD10 |
|---|---|
| Gene Synonyms | BIMP1 IMD89 CARMA3 |
| Gene Full Name | caspase recruitment domain family member 10 |
| Gene Summary | The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008] |
| Alternative Names |
CARD10 CARMA3 Caspase recruitment domain-containing protein 10 CARD-containing MAGUK protein 3 Carma 3 |
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cytoplasm . |
| SwissProt ID | Q9BWT7 |
|---|
WB
Western blot analysis of lysates from HeLa and 293 cells, using CARD10 Antibody. The lane on the right is blocked with the synthesized peptide.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4℃ overnight.3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
ICC/IF
Immunofluorescence analysis of HeLa cells, using CARD10 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
