WB
Western blot analysis of PGRMC1 using anti-PGRMC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PGRMC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PGRMC1 using anti-PGRMC1 antibody. PGRMC1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PGRMC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of PGRMC1 using anti-PGRMC1 antibody. PGRMC1 was detected in a paraffin-embedded section of human intestine cancer tissue. The tissue section was incubated with rabbit anti-PGRMC1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to PGRMC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PGRMC1, identical to the related mouse sequence, and different from the related rat sequence by two amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:8000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PGRMC1 |
|---|---|
| Gene Synonyms | IZA MPR Dap1 HPR6.6 |
| Gene Full Name | progesterone receptor membrane component 1 |
| Gene Summary | This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney. [provided by RefSeq, Mar 2010] |
| Alternative Names | HPR6.6 MPR PGRMC PGRMC1 |
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane,Microsome,Mitochondrion,Mitochondrion outer membrane,Secreted. |
| SwissProt ID | O00264 |
|---|
WB
Western blot analysis of PGRMC1 using anti-PGRMC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PGRMC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PGRMC1 using anti-PGRMC1 antibody. PGRMC1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-PGRMC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of PGRMC1 using anti-PGRMC1 antibody. PGRMC1 was detected in a paraffin-embedded section of human intestine cancer tissue. The tissue section was incubated with rabbit anti-PGRMC1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:1000-1:8000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
