WB
Western blot analysis of various lysates, using USP25 Rabbit mAb at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using USP25 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat kidney using USP25 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IP
Immunoprecipitation of USP25 from 300 µg extracts of Mouse testis was performed using 0.5 µg of USP25 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using USP25 Rabbit mAb at a dilution of 1:5000.| Product Name | USP25 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000 - 1:60000 IHC:1:50 - 1:20 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | USP25 |
|---|---|
| Gene Synonyms | EIG19 USP21 |
| Gene Full Name | ubiquitin specific peptidase 25 |
| Gene Summary | Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008] |
| Alternative Names | USP21 USP25 |
| Molecular Weight(MW) | 122KDa/125KDa/129KDa(Observed band size: 130kDa/130KD) |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q9UHP3 |
|---|
WB
Western blot analysis of various lysates, using USP25 Rabbit mAb at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using USP25 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat kidney using USP25 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation of USP25 from 300 µg extracts of Mouse testis was performed using 0.5 µg of USP25 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using USP25 Rabbit mAb at a dilution of 1:5000.| Application Notes | WB:1:2000 - 1:60000 IHC:1:50 - 1:20 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
