WB
Western blot analysis of INF2 using anti-INF2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-INF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of INF2 using anti-INF2 antibody. INF2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of human intestine cancer tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of INF2 using anti-INF2 antibody in Hela whole cell lysate. Western blot analysis of INF2 using anti- INF2 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- INF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of HEL cells using anti-INF2 antibody. Overlay histogram showing HEL cells stained with Anti-INF2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INF2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to INF2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human INF2 recombinant protein (Position: A7-Q674). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCIPWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INF2 |
|---|---|
| Gene Synonyms | FSGS5 CMTDIE pp9484 C14orf151 C14orf173 |
| Gene Full Name | inverted formin 2 |
| Gene Summary | This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.[provided by RefSeq, Aug 2010] |
| Alternative Names | C14orf151 C14orf173 HBEBP2 binding protein C INF2 Inverted formin 2 pp9484 |
| Molecular Weight(MW) | 136 kDa(Observed band size: 180 kDa) |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q27J81 |
|---|
WB
Western blot analysis of INF2 using anti-INF2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-INF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of INF2 using anti-INF2 antibody. INF2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of human intestine cancer tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of INF2 using anti-INF2 antibody. INF2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-INF2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of INF2 using anti-INF2 antibody in Hela whole cell lysate. Western blot analysis of INF2 using anti- INF2 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- INF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of HEL cells using anti-INF2 antibody. Overlay histogram showing HEL cells stained with Anti-INF2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INF2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
