WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-4E-BP1 (Phospho Thr37/46) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.ICC/IF
Immunofluorescence analysis of HeLa . Picture A: 4E-BP1 (Phospho 4E-BP1 (Phospho Thr37/46)) Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | 4E-BP1 (Phospho Thr37/46) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Thr37/Thr46 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF4EBP1 |
|---|---|
| Gene Synonyms | BP-1 4EBP1 4E-BP1 PHAS-I |
| Gene Full Name | eukaryotic translation initiation factor 4E binding protein 1 |
| Gene Summary | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008] |
| Alternative Names | EIF4EBP1 Eukaryotic translation initiation factor 4E-binding protein 1 4E-BP1 eIF4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 PHAS-I |
| Molecular Weight(MW) | 13 kDa(Observed band size: 15-20 kDa) |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13541 |
|---|
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-4E-BP1 (Phospho Thr37/46) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
ICC/IF
Immunofluorescence analysis of HeLa . Picture A: 4E-BP1 (Phospho 4E-BP1 (Phospho Thr37/46)) Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
