Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Raptor (Phospho Ser792) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Alternative Names
Regulatory-associated protein of mTOR
Raptor
p150 target of rapamycin
TOR
-scaffold protein
Molecular Weight(MW)
149 kDa(Observed band size: 150 kDa)
Cellular Localization
Cytoplasm. Lysosome. Cytoplasmic granule . Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells , accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Raptor (Phospho Ser792) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.