WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Raptor antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human colon was stained with anti-Raptor rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Raptor antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Raptor Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPTOR |
|---|---|
| Gene Synonyms | KOG1 Mip1 |
| Gene Full Name | regulatory associated protein of MTOR complex 1 |
| Gene Summary | This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Molecular Weight(MW) | 149 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q8N122 |
|---|
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Raptor antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human colon was stained with anti-Raptor rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Raptor antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
