WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FAK (Phospho Tyr576/Tyr577) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Product Name | FAK (Phospho Tyr576/Tyr577) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Tyr576/Tyr577 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000-1:50000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PTK2 |
|---|---|
| Gene Synonyms | FAK FADK FAK1 FRNK FADK 1 PPP1R71 p125FAK pp125FAK |
| Gene Full Name | protein tyrosine kinase 2 |
| Gene Summary | This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017] |
| Alternative Names |
PTK2 FAK FAK1 Focal adhesion kinase 1 FADK 1 Focal adhesion kinase-related nonkinase FRNK Protein phosphatase 1 regulatory subunit 71 PPP1R71 Protein-tyrosine kinase 2 p125FAK pp125FAK |
| Molecular Weight(MW) | 119 kDa(Observed band size: 125 kDa) |
| Cellular Localization | Cell junction , focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm , cell cortex. Cytoplasm , cytoskeleton. Cytoplasm , cytoskeleton , microtubule organizing center , centrosome . Nucleus. Cytoplasm , cytoskeleton , cilium basal body . Constituent of focal adhesions. Detected at microtubules. |
| SwissProt ID | Q05397 |
|---|
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FAK (Phospho Tyr576/Tyr577) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Application Notes | WB:1:5000-1:50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
