WB
Western blot analysis of Bid on different lysates with Rabbit anti-Bid antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IP
Bid was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with Bid Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Bid Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input). Lane 2: Bid Recombinant Rabbit Monoclonal Antibody IP in RAW264.7 cell lysate. Lane 3: Rabbit IgG instead of Bid Recombinant Rabbit Monoclonal Antibody in RAW264.7 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 46 seconds.| Product Name | Bid Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse Bid aa 51-195. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:5000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BID |
|---|---|
| Gene Synonyms | FP497 |
| Gene Full Name | BH3 interacting domain death agonist |
| Gene Summary | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020] |
| Alternative Names | Apoptic death agonist antibody Apoptotic death agonist BID antibody BH3 interacting domain death agonist antibody BH3 interacting domain death agonist p11 antibody BH3 interacting domain death agonist p13 antibody BH3 interacting domain death agonist p15 antibody BH3-interacting domain death agonist p11 antibody BID antibody BID isoform ES(1b) antibody BID isoform L(2) antibody BID isoform Si6 antibody BID_HUMAN antibody Desmocollin type 4 antibody FP497 antibody Human BID coding sequence antibody MGC15319 antibody MGC42355 antibody p11 BID antibody p13 BID antibody p15 BID antibody p22 BID antibody |
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm, Mitochondrion membrane, Mitochondrion outer membrane. |
| SwissProt ID | P55957 |
|---|
WB
Western blot analysis of Bid on different lysates with Rabbit anti-Bid antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IP
Bid was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with Bid Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Bid Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input). Lane 2: Bid Recombinant Rabbit Monoclonal Antibody IP in RAW264.7 cell lysate. Lane 3: Rabbit IgG instead of Bid Recombinant Rabbit Monoclonal Antibody in RAW264.7 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 46 seconds.| Application Notes | WB:1:5000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
