WB
Western blot analysis of CCR1 using anti-CCR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CCR1 using anti-CCR1 antibody. CCR1 was detected in a paraffin-embedded section of mouse lymphaden tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of CCR1 using anti-CCR1 antibody. CCR1 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of RAW264.7 cells using anti-CCR1 antibody.Overlay histogram showing RAW264.7 cells stained with Anti-CCR1 Antibody (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR1 Antibody (1:100) for 30 min at 20°C. 488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to CCR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCR1, different from the related human sequence by nine amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ccr1 |
|---|---|
| Gene Synonyms | Cmkbr1 Mip-1a-R |
| Gene Full Name | C-C motif chemokine receptor 1 |
| Gene Summary | Enables C-C chemokine receptor activity. Involved in negative regulation of innate immune response and positive regulation of neuroinflammatory response. Acts upstream of or within inflammatory response; leukocyte chemotaxis; and myeloid cell differentiation. Predicted to be located in plasma membrane. Predicted to be active in cytoplasm and external side of plasma membrane. Is expressed in aorta-gonad-mesonephros; extraembryonic component; limb; and liver. Used to study Coronavirus infectious disease. Human ortholog(s) of this gene implicated in allergic contact dermatitis and rheumatoid arthritis. Orthologous to human CCR1 (C-C motif chemokine receptor 1). [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | C-C motif chemokine receptor 1 CD191 CKR-1 CKR1 CMKBR1 HM145 MIP1aR SCYAR1 CCR1 |
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Cell membrane,Membrane. |
| SwissProt ID | P51675 |
|---|
WB
Western blot analysis of CCR1 using anti-CCR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CCR1 using anti-CCR1 antibody. CCR1 was detected in a paraffin-embedded section of mouse lymphaden tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of CCR1 using anti-CCR1 antibody. CCR1 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of RAW264.7 cells using anti-CCR1 antibody.Overlay histogram showing RAW264.7 cells stained with Anti-CCR1 Antibody (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR1 Antibody (1:100) for 30 min at 20°C. 488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
