WB
Primary: Anti-5HT2A Receptor at 1/300 dilution.
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IF-P
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5HT2A Receptor Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5HT2A Receptor Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5HT2A Receptor Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.