WB
Western blot analysis of UTX/KDM6A using anti-UTX/KDM6A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UTX/KDM6A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of UTX/KDM6A using anti-UTX/KDM6A antibody. UTX/KDM6A was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-UTX/KDM6A Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The tissue section was developed using Phalloidin-iFluor 488 Conjugated.FC
Flow Cytometry analysis of Hela cells using anti-UTX/KDM6A antibody. Overlay histogram showing Hela cells stained with Anti-UTX/KDM6A Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UTX/KDM6A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to UTX/KDM6A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human UTX/KDM6A recombinant protein (Position: A391-A704). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM6A |
|---|---|
| Gene Synonyms | UTX KABUK2 bA386N14.2 |
| Gene Full Name | lysine demethylase 6A |
| Gene Summary | This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014] |
| Alternative Names | UTX KDM6A histone H3 Histone demethylase UTX |
| Molecular Weight(MW) | 154 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O15550 |
|---|
WB
Western blot analysis of UTX/KDM6A using anti-UTX/KDM6A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UTX/KDM6A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of UTX/KDM6A using anti-UTX/KDM6A antibody. UTX/KDM6A was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-UTX/KDM6A Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The tissue section was developed using Phalloidin-iFluor 488 Conjugated.
FC
Flow Cytometry analysis of Hela cells using anti-UTX/KDM6A antibody. Overlay histogram showing Hela cells stained with Anti-UTX/KDM6A Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UTX/KDM6A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
