WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NGAL antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human spleen was stained with anti-NGAL Rabbit antibody.| Product Name | NGAL Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:1000-1:4000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LCN2 |
|---|---|
| Gene Synonyms | p25 24p3 MSFI NGAL |
| Gene Full Name | lipocalin 2 |
| Gene Summary | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015] |
| Alternative Names |
Neutrophil gelatinase-associated lipocalin NGAL 25 kDa alpha-2-microglobulin-related subunit of MMP-9 Lipocalin-2 Oncogene 24p3 Siderocalin LCN2 p25 |
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Secreted . Cytoplasmic granule lumen . Cytoplasmic vesicle lumen . Upon binding to the SLC22A17 (24p3R) receptor , it is internalized (By similarity) . Releases the bound iron in the acidic lumen of cytoplasmic vesicles (PubMed:12453413 , PubMed:20581821) . |
| SwissProt ID | P80188 |
|---|
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NGAL antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human spleen was stained with anti-NGAL Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
