WB
mouse liver tissue were subjected to SDS PAGE followed by western blot with CD31 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
mouse spleen tissue were subjected to SDS PAGE followed by western blot with CD31 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using CD31 antibody at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Cd31 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using CD31 antibody at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | CD31 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant mouse Pecam1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:1000-1:8000 IHC:1:4000-1:16000 IF-P:1:200-1:800 IF-F:1:200-1:800 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pecam1 |
|---|---|
| Gene Synonyms | Cd31 Pecam PECAM-1 |
| Gene Full Name | platelet/endothelial cell adhesion molecule 1 |
| Gene Summary | Predicted to enable several functions, including protein homodimerization activity; protein phosphatase binding activity; and receptor ligand activity. Acts upstream of or within several processes, including Rho protein signal transduction; endothelial cell morphogenesis; and positive regulation of tyrosine phosphorylation of STAT protein. Located in several cellular components, including cell-cell contact zone; membrane raft; and smooth muscle contractile fiber. Is active in external side of plasma membrane. Is expressed in several structures, including alimentary system; cardiovascular system; central nervous system; extraembryonic component; and genitourinary system. Human ortholog(s) of this gene implicated in coronary artery disease; coronary stenosis; myocardial infarction; neuroblastoma; and psoriatic arthritis. Orthologous to human PECAM1 (platelet and endothelial cell adhesion molecule 1). [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | Pecam PECAM 1 Pecam1 PECAM-1 |
| Molecular Weight(MW) | 81 kDa(Observed band size: 110-130 kDa) |
| Cellular Localization | Cell junction,Cell membrane,Membrane |
WB
mouse liver tissue were subjected to SDS PAGE followed by western blot with CD31 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
mouse spleen tissue were subjected to SDS PAGE followed by western blot with CD31 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using CD31 antibody at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Cd31 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using CD31 antibody at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:8000 IHC:1:4000-1:16000 IF-P:1:200-1:800 IF-F:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
