WB
Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes. Negative expression of Osteoprotegerin/TNFRSF11B protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.FC
Application: Flow Cytometry (Intra). Species: Human. Sample: K-562 (Human chronic myelogenous leukemia cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody:Osteoprotegerin Recombinant Rabbit Monoclonal Antibody. (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Kidney. Sample: Frozen section. Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Kidney. Sample: Frozen section. Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.| Product Name | Osteoprotegerin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse Osteoprotegerin aa 1-401. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-FIHCWB |
| WB:1:5000 IHC:1:200-1:1000 FC:1:1000 IF-F:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFRSF11B |
|---|---|
| Gene Synonyms | OPG TR1 OCIF PDB5 |
| Gene Full Name | TNF receptor superfamily member 11b |
| Gene Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | MGC29565 antibody OCIF antibody OPG antibody Osteoclastogenesis inhibitory factor antibody Osteoprotegerin antibody PDB5 antibody TNF receptor superfamily member 11b antibody TNFRSF 11B antibody TNFRSF11B antibody TR 1 antibody TR1 antibody TR11B_HUMAN antibody Tumor necrosis factor receptor superfamily member 11B antibody |
| Molecular Weight(MW) | 46 kDa(Observed band size: 56 kDa) |
| Cellular Localization | Secreted. |
| SwissProt ID | O00300 |
|---|
WB
Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes. Negative expression of Osteoprotegerin/TNFRSF11B protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
FC
Application: Flow Cytometry (Intra). Species: Human. Sample: K-562 (Human chronic myelogenous leukemia cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody:Osteoprotegerin Recombinant Rabbit Monoclonal Antibody. (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Kidney. Sample: Frozen section. Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Kidney. Sample: Frozen section. Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Osteoprotegerin Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.| Application Notes | WB:1:5000 IHC:1:200-1:1000 FC:1:1000 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
