WB
Figure. Western Blot; Sample: Recombinant PLCe1, Rat.WB
Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat PLCe1 Antibody. Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.IHC
Used in DAB staining on fromalin fixed paraffin- embedded testis tissue.| Product Name | Polyclonal Antibody to Phospholipase C Epsilon 1 (PLCe1) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant PLCe1 (Met1~Lys250) expressed in E.coli. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:0.5-2µg/mL IHC:5-20µg/mL |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Symbol | PLCE1 |
|---|---|
| Gene Synonyms | PLCE PPLC NPHS3 |
| Gene Full Name | phospholipase C epsilon 1 |
| Gene Summary | This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009] |
| Alternative Names | PLCE NPHS3 PPLC Phosphoinositide phospholipase C-epsilon-1 Pancreas-enriched phospholipase C 1-phosphatidylinositol 4 5-bisphosphate phosphodiesterase epsilon-1 |
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Golgi apparatus,Membrane. |
| SwissProt ID | Q9P212 |
|---|
WB
Figure. Western Blot; Sample: Recombinant PLCe1, Rat.
WB
Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat PLCe1 Antibody. Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
IHC
Used in DAB staining on fromalin fixed paraffin- embedded testis tissue.| Application Notes | WB:0.5-2µg/mL IHC:5-20µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. |
| Storage Buffer | Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
