WB
Western blot analysis of ERF using anti-ERF antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ERF using anti-ERF antibody. ERF was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-ERF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to ERF |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ERF recombinant protein (Position: E308-S548). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERF |
|---|---|
| Gene Synonyms | PE2 CRS4 PE-2 CHYTS |
| Gene Full Name | ETS2 repressor factor |
| Gene Summary | ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] |
| Alternative Names | PE2 PE 2 Ets2 repressor factor |
| Molecular Weight(MW) | 59 kDa(Observed band size: 70-100 kDa) |
| Cellular Localization | Nucleus. |
| SwissProt ID | P50548 |
|---|
WB
Western blot analysis of ERF using anti-ERF antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ERF using anti-ERF antibody. ERF was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-ERF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
