WB
Western blot analysis of NEK7 expression in HepG2 (A), Jurkat (B), mouse heart (C) whole cell lysates.IHC
Immunohistochemical analysis of NEK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Anti-NEK7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NEK7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:100 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEK7 |
|---|---|
| Gene Full Name | NIMA related kinase 7 |
| Gene Summary | NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002] |
| Alternative Names | Serine/threonine-protein kinase Nek7 Never in mitosis A-related kinase 7 NimA-related protein kinase 7 |
| Molecular Weight(MW) | 34 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Cytoplasm,Cytoskeleton,Microtubule,Nucleus. |
| SwissProt ID | Q8TDX7 |
|---|
WB
Western blot analysis of NEK7 expression in HepG2 (A), Jurkat (B), mouse heart (C) whole cell lysates.
IHC
Immunohistochemical analysis of NEK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:1000 IHC:1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
