WB
Western blot analysis of Dopamine Transporter on different lysates with Rabbit anti-Dopamine Transporter antibody at 1/2,000 dilution. Notice: no heat means the lysate is not boiled. Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Dopamine Transporter antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Dopamine Transporter antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Application: IHC-Fr. Species: Mouse. Site: Striatum. Sample: Frozen section. Antibody concentration: 1:500. Antigen retrieval: Not required.IF-F
Application: IHC-Fr. Species: Rat. Site: Striatum. Sample: Frozen section. Antibody concentration: 1:500. Antigen retrieval: Not required.| Product Name | Dopamine Transporter Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:2000 IHC:1:500-1:1000 IF-F:1:500-1:1000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC6A3 |
|---|---|
| Gene Synonyms | DAT DAT1 PKDYS PKDYS1 |
| Gene Full Name | solute carrier family 6 member 3 |
| Gene Summary | This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009] |
| Alternative Names | DA transporter antibody DAT 1 antibody DAT antibody DAT1 antibody Dopamine transporter 1 antibody Dopamine transporter antibody PKDYS antibody SC6A3_HUMAN antibody SLC6A3 antibody Sodium dependent dopamine transporter antibody Sodium-dependent dopamine transporter antibody Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody Solute carrier family 6 (neurotransmitter transporter) member 3 antibody Solute carrier family 6 member 3 antibody Variable number tandem repeat (VNTR) antibody |
| Molecular Weight(MW) | 69 kDa(Observed band size: 75 kDa) |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q61327 |
|---|
WB
Western blot analysis of Dopamine Transporter on different lysates with Rabbit anti-Dopamine Transporter antibody at 1/2,000 dilution. Notice: no heat means the lysate is not boiled. Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Dopamine Transporter antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Dopamine Transporter antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Application: IHC-Fr. Species: Mouse. Site: Striatum. Sample: Frozen section. Antibody concentration: 1:500. Antigen retrieval: Not required.
IF-F
Application: IHC-Fr. Species: Rat. Site: Striatum. Sample: Frozen section. Antibody concentration: 1:500. Antigen retrieval: Not required.| Application Notes | WB:1:2000 IHC:1:500-1:1000 IF-F:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
