WB
Western blot analysis of GSTK1 using anti-GSTK1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSTK1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of GSTK1 using anti-GSTK1 antibody in 293T whole cell lysate. Western blot analysis of GSTK1 using anti- GSTK1 antibody. Lane 1: 293T whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- GSTK1 antibody in 293T whole cell lysate, Lane 3: anti- GSTK1 antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- GSTK1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of HepG2 cells using anti-GSTK1 antibody. Overlay histogram showing HepG2 cells stained with Anti-GSTK1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSTK1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to GSTK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GSTK1 recombinant protein (Position: N53-K213). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSTK1 |
|---|---|
| Gene Synonyms | GST GST13 hGSTK1 GSTK1-1 GST13-13 GST 13-13 |
| Gene Full Name | glutathione S-transferase kappa 1 |
| Gene Summary | This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] |
| Alternative Names | DsbA-L GST 13 13 GST Glutathione S-transferase subunit 13 Glutathione S-transferase kappa 1 |
| Molecular Weight(MW) | 25 kDa(Observed band size: 26 kDa) |
| Cellular Localization | Peroxisome. |
| SwissProt ID | Q9Y2Q3 |
|---|
WB
Western blot analysis of GSTK1 using anti-GSTK1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSTK1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of GSTK1 using anti-GSTK1 antibody. GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GSTK1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of GSTK1 using anti-GSTK1 antibody in 293T whole cell lysate. Western blot analysis of GSTK1 using anti- GSTK1 antibody. Lane 1: 293T whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- GSTK1 antibody in 293T whole cell lysate, Lane 3: anti- GSTK1 antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- GSTK1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of HepG2 cells using anti-GSTK1 antibody. Overlay histogram showing HepG2 cells stained with Anti-GSTK1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSTK1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 IP:1:250-1:300 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
