IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using PSD95 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded Human stomach tissues using PSD95 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded Human brain tissues using PSD95 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-PSD95 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC |
| IHC:1:200-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DLG4 |
|---|---|
| Gene Synonyms | A330103J02Rik B130052P05Rik B230218P12Rik B330007M19Rik chapsyn-110 DLGH1 Dlgh2 Dlgh3 Dlgh4 E-dlg/SAP97 fa66c08 fa66e02 fd02c12 fu95c12 Gm1197 Gm21505 hdlg im:7138640 mKIAA1232 mKIAA4187 MRD62 MRX MRX90 NEDLG PPP1R58 PPP1R82 PSD-93 PSD-95 PSD93 PSD95 SAP-90 SAP-97 SAP-97A SAP102 SAP90 SAP90A SAP97 si:ch211-276g21.1 wu:fa66c08 wu:fa66e02 wu:fc34d02 wu:fd02c12 wu:fu95c12 XLID90 XLMR zgc:55810 |
| Gene Full Name | discs large MAGUK scaffold protein 4 |
| Gene Summary | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | channel associated protein of synapse-110 channel-associated protein of synapse-110 channel-associated protein of synapses 110kDa chapsyn-110 discs large homolog 1 scribble cell polarity complex component discs large homolog 4 discs large MAGUK scaffold protein 2 eta discs large MAGUK scaffold protein 2 gamma discs large homolog 1 discs large homolog 2 discs large homolog 2 chapsyn-110 discs large homolog 3 discs large homolog 4 disks large 1 tumor suppressor protein-like dJ1061C18.1.1 DLG1 E-dlg/SAP97 Drosophila discs-large tumor suppressor homologue (synapse associated protein) embryo-dlg/synapse-associated protein 97 membrane associated guanylate kinase MAGUK neuroendocrine-DLG post-synaptic density protein 95 postsynaptic density protein 93 postsynaptic density protein 95 postsynaptic density protein PSD-93 presynaptic protein SAP97 protein phosphatase 1 regulatory subunit 58 protein phosphatase 1 regulatory subunit 82 PSD-93 protein PSD-95 PSD-95 alpha 2b PSD-95 beta PSD-95/SAP90-related protein 1 SAP-102 SAP-90 SAP102 SAP97A synapse-associated protein 102 synapse-associated protein 90 synapse-associated protein 97 synapse-associated protein 97A synapse-associated protein SAP90 synaptic density protein PSD-93 Tax interaction protein 15 |
| Source | Rabbit |
| Cellular Localization | Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse |
| SwissProt ID | P78352 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using PSD95 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human stomach tissues using PSD95 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human brain tissues using PSD95 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | IHC:1:200-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
