IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues tissues using SPARC antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using SPARC antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using SPARC antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using SPARC antibody with DAB staining.WB
Western blot analysis using SPARC antibody against Hela(1),Hepg2(2),Cos-7(3),Nih3t3(4),Mcf-7(5) cell lysate.| Product Name | Anti-SPARC antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPARC |
|---|---|
| Gene Synonyms | ON ONT OI17 BM-40 |
| Gene Full Name | secreted protein acidic and cysteine rich |
| Gene Summary | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. |
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Basement membrane, Extracellular matrix, Secreted |
| SwissProt ID | P09486 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues tissues using SPARC antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using SPARC antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using SPARC antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using SPARC antibody with DAB staining.
WB
Western blot analysis using SPARC antibody against Hela(1),Hepg2(2),Cos-7(3),Nih3t3(4),Mcf-7(5) cell lysate.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
