ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using Peroxiredoxin 6 antibody against Hela(1),Hepg2(2),K562(3),A549(4)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Peroxiredoxin 6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRDX6 |
|---|---|
| Gene Synonyms | 1-Cys 1-Cys Prx 1-cysPrx 9430088D19Rik aiPLA2 AOP2 Brp-12 CP-3 GPx HEL-S-128m LPCAT-5 Ltw-4 Ltw4 Lvtw-4 NSGPx ORF06 p29 Prdx5 PRX |
| Gene Full Name | peroxiredoxin 6 |
| Gene Summary | The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1-Cys peroxiredoxin 1-Cys PRX 24 kDa protein acidic calcium-independent phospholipase A2 aiPLA2 anti-oxidant protein 2 related sequence 3 antioxidant antioxidant protein 2 epididymis secretory sperm binding protein Li 128m glutathione-dependent peroxiredoxin KIAA0106 liver 2D page spot 40 LPC acyltransferase 5 lyso-PC acyltransferase 5 lysophosphatidylcholine acyltransferase 5 MGC46173 MSP23 NKEFA non-selenium glutathione peroxidase NSGPx PAG PAGA PAGB peroxiredoxin 5 related sequence 3 peroxiredoxin 6 related sequence 3 Prx6 red blood cells page spot 12 thiol-specific antioxidant protein |
| Molecular Weight(MW) | 25kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm,Lysosome |
| SwissProt ID | P30041 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using Peroxiredoxin 6 antibody against Hela(1),Hepg2(2),K562(3),A549(4)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
