WB
Western blot analysis of PRDX6 using anti-PRDX6 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PRDX6 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PRDX6 using anti-PRDX6 antibody. PRDX6 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-PRDX6 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of PRDX6 using anti-PRDX6 antibody. PRDX6 was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-PRDX6 Antibody at a dilution of 1:100. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Hela cells using anti-PRDX6 antibody. Overlay histogram showing Hela cells stained with Anti-PRDX6 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PRDX6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Mouse monoclonal antibody to PRDX6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6,respectively. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Symbol | PRDX6 |
|---|---|
| Gene Synonyms | PRX p29 AOP2 1-Cys NSGPx aiPLA2 LPCAT-5 HEL-S-128m |
| Gene Full Name | peroxiredoxin 6 |
| Gene Summary | The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1 Cys 1 Cys peroxiredoxin 1 Cys PRX 24 kDa protein aiPLA2 Antioxidant protein 2 AOP2 KIAA0106 Liver 2D page spot 40 NSGPx p29 peroxiredoxin 6 PRDX6 PRX Red blood cells page spot 12 |
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm,Lysosome. |
WB
Western blot analysis of PRDX6 using anti-PRDX6 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-PRDX6 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PRDX6 using anti-PRDX6 antibody. PRDX6 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-PRDX6 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of PRDX6 using anti-PRDX6 antibody. PRDX6 was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-PRDX6 Antibody at a dilution of 1:100. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Hela cells using anti-PRDX6 antibody. Overlay histogram showing Hela cells stained with Anti-PRDX6 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PRDX6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
