| Product Name | Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 |
|---|---|
| Antibody Type | Secondary Antibodies |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugation | Alexa Fluor™ 555 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Donkey |
| Tested Applications | ICC/IFWBFlowIHC-P |
| ICC/IF:1-2 µg/mL WB:0.2-2 µg/mL Flow:1-4 µg/mL IHC-P:1:2,000 |
|
| Species Reactivity | Rat |
| Predicted Reactivity | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep serum |
| Concentration | 2mg/ml |
| Purification | Antigen affinity chromatography |
| Target Description | Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
|---|---|
| Function | 553/568 nm |
| Application Notes | ICC/IF:1-2 µg/mL WB:0.2-2 µg/mL Flow:1-4 µg/mL IHC-P:1:2,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C, store in dark, DO NOT FREEZE! |
| Storage Buffer | PBS, pH 7.5;5mM sodium azide |
