| Product Name | Donkey anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 594 |
|---|---|
| Antibody Type | Secondary Antibodies |
| Immunogen | Human IgG-heavy and light chain |
| Conjugation | DyLight™ 594 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Donkey |
| Tested Applications | ICC/IFWBFlowIHC |
| ICC/IF:1:50-1:500 WB:1:2,000-1:20,000 Flow:1:50-1:200 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Against bovine, chicken, goat, mouse, rabbit, rat and sheep |
| Concentration | 0.5mg/ml |
| Purification | Antigen affinity chromatography |
| Target Description | Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
|---|---|
| Function | 587/614 nm |
| Application Notes | ICC/IF:1:50-1:500 WB:1:2,000-1:20,000 Flow:1:50-1:200 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C |
| Storage Buffer | PBS, pH 6.8 to 7.4, with 0.2% BSA;0.09% sodium azide |
