| Product Name | Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin |
|---|---|
| Antibody Type | Secondary Antibodies |
| Conjugation | Biotin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Donkey |
| Tested Applications | ICC/IFWBFlowIHCELISAIP |
| ICC/IF:1:500-1:5,000 WB:1:20,000-1:400,000 Flow:1:200-1:1,000 IHC:1:500-1:5,000 ELISA:1:20,000-1:400,000 IP:1:500-1:5,000 |
|
| Species Reactivity | Rabbit |
| Predicted Reactivity | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins |
| Concentration | 1mg/ml |
| Purification | Antigen affinity chromatography |
| Target Description | Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
|---|
| Application Notes | ICC/IF:1:500-1:5,000 WB:1:20,000-1:400,000 Flow:1:200-1:1,000 IHC:1:500-1:5,000 ELISA:1:20,000-1:400,000 IP:1:500-1:5,000 |
|---|
| Form | Lyophilized |
|---|---|
| Storage Instructions | 4°C |
| Storage Buffer | PBS, pH 7.6, with 15mg/mL BSA;0.05% sodium azide |
