| Product Name | Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin |
|---|---|
| Antibody Type | Secondary Antibodies |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugation | Biotin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Donkey |
| Tested Applications | ICC/IFWBFlowELISA |
| ICC/IF:1:500-1:20,000 WB:1:500-1:20,000 Flow:1:500-1:20,000 ELISA:1:500-1:20,000 |
|
| Species Reactivity | Mouse |
| Concentration | 1.5mg/ml |
| Purification | Affinity chromatography |
| Target Description | Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
|---|
| Application Notes | ICC/IF:1:500-1:20,000 WB:1:500-1:20,000 Flow:1:500-1:20,000 ELISA:1:500-1:20,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Storage Buffer | PBS, pH 7.2, with 1% BSA;0.05% sodium azide |
