| Product Name | Rabbit anti-Mouse IgG (Heavy Chain) Secondary Antibody, Alexa Fluor™ 488 |
|---|---|
| Antibody Type | Secondary Antibody |
| Immunogen | Recombinant full-length protein |
| Conjugation | Alexa Fluor™ 488Alexa Fluor™ 488Alexa Fluor™ 488查看其它偶联染料Alexa Fluor 350Alexa Fluor 555Alexa Fluor 555Alexa Fluor 568Alexa Fluor 594Alexa Fluor 633Alexa Fluor 647Alexa Fluor 647Alexa Fluor 680Alexa Fluor Plus 488Alexa Fluor Plus 555Alexa Fluor Plus 594Alexa Fluor Plus 647APAPBiotinBiotinDyLight 488DyLight 550DyLight 650DyLight 680DyLight 755DyLight 800FITCFITCHRPHRPHRPRhodamineTRITCUnconjugatedUnconjugatedRequest custom conjugation |
| Clonality | Recombinant Superclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFFlowWB |
| ICC/IF:1:1,000-1:2,000 Flow:1-3 µg/mL WB:1:2,500-1:5,000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Gravity column chromatography |
| Target | IgG (Heavy chain) |
|---|---|
| Target Description | Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
| Function | 499/520 nm |
| Application Notes | ICC/IF:1:1,000-1:2,000 Flow:1-3 µg/mL WB:1:2,500-1:5,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C, store in dark, DO NOT FREEZE! |
| Storage Buffer | PBS, pH 7.2-7.4;5mM sodium azide |
