| Product Name | Rabbit anti-Human IgG (H+L) Secondary Antibody, AP |
|---|---|
| Antibody Type | Secondary Antibody |
| Immunogen | Human IgG whole molecule. |
| Conjugation | APAPAP查看其它偶联染料BiotinDyLight 488DyLight 550DyLight 594DyLight 650DyLight 680DyLight 755DyLight 800FITCHRPTexas RedTRITCUnconjugatedRequest custom conjugation |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWBELISA |
| IHC:1:200-1:1,000 WB:1:500-1:2,000 ELISA:1:2,000-1:10,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Antigen affinity chromatography |
| Target | IgG |
|---|---|
| Target Description | Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
| Application Notes | IHC:1:200-1:1,000 WB:1:500-1:2,000 ELISA:1:2,000-1:10,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C, do not freeze |
| Storage Buffer | TBS, pH 8.0, with 1mM MgCl2, 1µM ZnCl2, 50% glycerol, 1% BSA;0.01% sodium azide |
