WB
Western blot analysis using SHC1 antibody against C2C12(1), Cos-7(2),Nih3t3(3),Mcf-7(4)cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using SHC1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using SHC1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using SHC1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using SHC1 antibody with DAB staining.| Product Name | Anti-SHC1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SHC1 |
|---|---|
| Gene Synonyms | SHC SHCA |
| Gene Full Name | SHC adaptor protein 1 |
| Gene Summary | This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| Molecular Weight(MW) | 63kDa |
| Cellular Localization | Cytoplasm, Mitochondrion |
| SwissProt ID | P29353 |
|---|
WB
Western blot analysis using SHC1 antibody against C2C12(1), Cos-7(2),Nih3t3(3),Mcf-7(4)cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using SHC1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using SHC1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using SHC1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using SHC1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
