IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using GAD2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using GAD2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-GAD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC |
| IHC:1:200-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GAD2 |
|---|---|
| Gene Synonyms | 6330404F12Rik CPSQ1 DEE89 EP10 GAD GAD(65) Gad-1 Gad-2 GAD-67 GAD65 GAD67 SCP zgc:112198 |
| Gene Full Name | glutamate decarboxylase 2 |
| Gene Summary | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| Alternative Names | 65 kD isoform 65 kDa glutamic acid decarboxylase 65kDa pancreatic beta cell autoantigen 67 kD isoform 67 kDa glutamic acid decarboxylase EC 4.1.1.15 FLJ45882 GAD GAD 1 GAD 2 GAD 65 GAD 67 GAD-65 GAD-67 GAD67 short splice variant gamma-aminobutyric acid biosynthesis enzyme glutamate decarboxylase 1 glutamate decarboxylase 1 (brain 67kDa) isoform GAD1_1 glutamate decarboxylase 1 (brain 67kDa) isoform GAD1_2 glutamate decarboxylase 1 (brain 67kDa) isoform GAD1_3 Glutamate decarboxylase 1 (brain) glutamate decarboxylase 1 (brain 67kDa) glutamate decarboxylase 1 variant GAD67NT Glutamate decarboxylase 2 (islet) glutamate decarboxylase 2 (pancreatic islets and brain 65kDa) glutamate decarboxylase 67 kDa isoform Glutamate decarboxylase-2 (pancreas) glutamic acid decarboxylase glutamic acid decarboxylase 1 glutamic acid decarboxylase 2 glutamic acid decarboxylase 65 |
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane |
| SwissProt ID | Q05329 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using GAD2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded Human Kidney tissues using GAD2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | IHC:1:200-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
