ICC/IF
Immunofluorescence analysis of A549 cells using CXCL12/SDF1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CXCL12/SDF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCL12 |
|---|---|
| Gene Synonyms | IRH PBSF SCYB12 SDF1 TLSF TPAR1 |
| Gene Full Name | C-X-C motif chemokine ligand 12 |
| Gene Summary | This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| Alternative Names | 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 AI174028 C Cmotif chemokine C X C motif chemokine CC motif chemokine CCmotif chemokine chemokine chemokine (C-X-C motif) ligand 12 CXC CXC motif chemokine CXCL CXCL12 hIRH hSDF-1 intercrine reduced in hepatomas interleukin-8 homolog pre-B cell growth-stimulating factor pre-B-cell growth-stimulating factor SDF-1 delta chemokine (C-X-C motif) ligand 12 transcript variant 5 SDF-1 epsilon chemokine (C-X-C motif) ligand 12 transcript variant 6 SDF-1 gamma chemokine (C-X-C motif) ligand 12 transcript variant 4 SDF-1 theta chemokine (C-X-C motif) ligand 12 transcript variant 7 SDF-1a SDF-1b SDF1a stromal cell-derived factor-1 alpha thymic lymphoma cell-stimulating factor TLSF-a TLSF-b Tlsfa Tlsfb |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Secreted |
| SwissProt ID | P48061 |
|---|
ICC/IF
Immunofluorescence analysis of A549 cells using CXCL12/SDF1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
