WB
Western blot analysis using NRAS antibody against Mouse serum (1),Mouse intestine(2),Mouse stomach(3),Rat intestine(4),Rat stomach(5) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-NRAS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NRAS |
|---|---|
| Gene Synonyms | C-K-RAS ALPS4 C-BAS/HAS C-H-RAS c-Ha-ras C-HA-RAS1 C-K-RAS c-Ki-ras c-Ki-ras2 c-rasHa CFC2 CMNS CTLO H-ras H-RASIDX Ha-ras HAMSV Harvey-ras Hras-1 HRAS1 K-Ras K-Ras 2 K-RAS2A K-RAS2B K-RAS4A K-RAS4B KI-RAS Kras-2 KRAS1 KRAS2 N-ras NCMS NRAS1 NS NS3 NS6 OES p21 p21B p21ras RALD RALD1 RALD2 ras RASH1 RASK2 |
| Gene Full Name | NRAS proto-oncogene, GTPase |
| Gene Summary | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. |
| Alternative Names | c-Ha-ras p21 protein c-Ha-ras transgene c-has/bas p21 protein c-K-ras c-K-ras2 protein c-Kirsten-ras protein c-Kirsten-ras proto-oncogene c-ras-Ki-2 protein cellular c-Ki-ras2 cellular c-Ki-ras2 proto-oncogene cellular transforming proto-oncogene GTP- and GDP-binding peptide B GTPase Hras1 H-ras 1 protein H-Ras-1 Ha-Ras1 proto-oncoprotein Harvey ras1 protein Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog Harvey rat sarcoma viral oncogene homolog Harvey rat sarcoma viral oncoprotein Harvey rat sarcoma virus oncogene Harvey rat sarcoma virus oncogene 1 HRAS K RAS K-Ras 2 K-ras p21 protein ki-Ras Kirsten rat sarcoma oncogene 2 expressed Kirsten rat sarcoma viral oncogene homolog Kirsten rat sarcoma viral oncogene homologue 2 (active) Kirsten rat sarcoma viral proto-oncogene KRAS-ENOPH fusion Kras-Enoph1 fusion protein N-ras protein part 4 Neuroblastoma RAS neuroblastoma ras oncogene neuroblastoma RAS viral (v-ras) oncogene homolog neuroblastoma RAS viral oncogene homolog Nras oncogene KRAS2 p19 H-RasIDX protein p21 protein p21ras PR310 c-K-ras oncogene PR371 c-K-ras oncogene proto-oncogene GTPase Ras family small GTP binding protein H-Ras RASK RASN small GTP binding protein transformation gene: oncogene HAMSV transforming protein N-Ras transforming protein P21 v-Ha-ras Harvey rat sarcoma viral oncogene homolog v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog v-ras neuroblastoma RAS viral oncogene homolog |
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Cell membrane, Golgi apparatus, Membrane |
| SwissProt ID | P01111 |
|---|
WB
Western blot analysis using NRAS antibody against Mouse serum (1),Mouse intestine(2),Mouse stomach(3),Rat intestine(4),Rat stomach(5) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
