WB
Western blot analysis using GRIA3 antibody against Mouse brain(1),Mouse testes(2),Mouse liver(3) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-GRIA3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GRIA3 |
|---|---|
| Gene Synonyms | 2900064I19Rik GluA2 GluA3 Glur-2 GluR-3 GluR-B GLUR-C GluR-K2 GLUR-K3 GLUR2 GLUR3 Gluralpha3 GLURB GLURC HBGR2 iGluR3 MRX94 MRXSW NEDLIB |
| Gene Full Name | glutamate ionotropic receptor AMPA type subunit 3 |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008] |
| Alternative Names | AMPA receptor subunit GluA2 AMPA receptor subunit GluA3 AMPA selective glutamate receptor AMPA-selective glutamate receptor 2 AMPA-selective glutamate receptor 3 dJ1171F9.1 gluR-3 GluR2 glutamate receptor glutamate receptor (GluR-B) glutamate receptor (GluR-C) glutamate receptor B glutamate receptor C glutamate receptor channel alpha3 subunit glutamate receptor ionotropic AMPA 2 glutamate receptor subunit 3 glutamate receptor ionotrophic AMPA 3 glutamate receptor ionotropic AMPA 2 glutamate receptor ionotropic AMPA 3 glutamate receptor ionotropic AMPA3 (alpha 3) GPRC1C GRM3 MGR3 |
| Molecular Weight(MW) | 101KD |
| Cellular Localization | Plasma membrane |
| SwissProt ID | P42263 |
|---|
WB
Western blot analysis using GRIA3 antibody against Mouse brain(1),Mouse testes(2),Mouse liver(3) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
