WB
Western blot analysis using Flag tag mouse monoclonal antibody against BSA-Flag and BSA lysates.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using Flag tag antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using Flag tag antibody with DAB staining.| Product Name | Anti-Flag tag antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | poly-HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | DDDDK epitope tag antibody DDDK antibody ddk antibody DYKDDDDK antibody DYKDDDDK epitope tag antibody DYKDDDDK tag antibody ECS epitope tag antibody ECS tag antibody Enterokinase Cleavage Site epitope tag antibody Enterokinase Cleavage Site tag antibody FLAG antibody FLAG tag antibody |
|---|---|
| Molecular Weight(MW) | 70kDa |
| Function | The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. |
| Cellular Localization | Cell membrane Cell membrane Cell membrane |
WB
Western blot analysis using Flag tag mouse monoclonal antibody against BSA-Flag and BSA lysates.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using Flag tag antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using Flag tag antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
