ICC/IF
Immunofluorescence analysis of U251 cells using Rubisco antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-Rubisco(Large Chain) antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | AF488 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IFFC |
| Species Reactivity | Plants |
| Purification | Affinity purified |
| Molecular Weight(MW) | 53kDa |
|---|---|
| Function | Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes. |
ICC/IF
Immunofluorescence analysis of U251 cells using Rubisco antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
