WB
Western blot analysis using HP-1α antibody against Huvec(1),Thp-1(2),Nih3t3(3),Mcf-7(4),Hela(5),Hepg2 (6),A549(7),Hek293(8),Jurkat(9),C2C12(10),Cos-7(11),Pc-12(12),K562(13),A431(14) lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using HP-1α antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using HP-1α antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HP-1α antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using HP-1α antibody with DAB staining.| Product Name | Anti-HP-1α antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | CBX5 |
|---|---|
| Gene Synonyms | HP1 HP1A HEL25 HP1alpha |
| Gene Full Name | chromobox 5 |
| Gene Summary | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 22kDa |
| Cellular Localization | Centromere,Chromosome,Nucleus. |
| SwissProt ID | P45973 |
|---|
WB
Western blot analysis using HP-1α antibody against Huvec(1),Thp-1(2),Nih3t3(3),Mcf-7(4),Hela(5),Hepg2 (6),A549(7),Hek293(8),Jurkat(9),C2C12(10),Cos-7(11),Pc-12(12),K562(13),A431(14) lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using HP-1α antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using HP-1α antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using HP-1α antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using HP-1α antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
