WB
Western blot analysis using Smad7 antibody against Huvec(1),Nih3t3(2),Mcf-7(3),Hela(4),Hepg2(5),A549(6),Hek293(7),Cos-7(8),K562(9,)A431(10) lysate .IHC
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Smad7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Smad7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Smad7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Smad7 antibody with DAB staining.| Product Name | Anti-Smad7 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | poly-HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | SMAD7 |
|---|---|
| Gene Synonyms | CRCS3 MADH7 MADH8 |
| Gene Full Name | SMAD family member 7 |
| Gene Summary | The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Molecular Weight(MW) | 46kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | O15105 |
|---|
WB
Western blot analysis using Smad7 antibody against Huvec(1),Nih3t3(2),Mcf-7(3),Hela(4),Hepg2(5),A549(6),Hek293(7),Cos-7(8),K562(9,)A431(10) lysate .
IHC
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Smad7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Smad7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Smad7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Smad7 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
