WB
Western blot analysis using Smad3 antibody against Hela(1),A549(2),Mcf-7(3),Mouse liver tissue(4),Mouse lung tissue(5),Mouse spleen tissue(6) lysate .IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using Smad3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Smad3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using Smad3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using Smad3 antibody with DAB staining.| Product Name | Anti-Smad3 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Conjugation | poly-HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | SMAD3 |
|---|---|
| Gene Synonyms | LDS3 mad3 LDS1C MADH3 JV15-2 hMAD-3 hSMAD3 HSPC193 HsT17436 |
| Gene Full Name | SMAD family member 3 |
| Gene Summary | The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, May 2022] |
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | P84022 |
|---|
WB
Western blot analysis using Smad3 antibody against Hela(1),A549(2),Mcf-7(3),Mouse liver tissue(4),Mouse lung tissue(5),Mouse spleen tissue(6) lysate .
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using Smad3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Smad3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using Smad3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using Smad3 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide.Purified antibody in PBS with 0.05% PC300. |
