ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Product Name | Anti-Phospho-Smad3 (Ser213) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser213 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD3 |
|---|---|
| Gene Synonyms | LDS3 mad3 LDS1C MADH3 JV15-2 hMAD-3 hSMAD3 HSPC193 HsT17436 |
| Gene Full Name | SMAD family member 3 |
| Gene Summary | The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, May 2022] |
| Alternative Names | DKFZP586N0721 DKFZp686J10186 hMAD 3 hMAD-3 hSMAD3 HSPC193 HST17436 JV15 2 JV15-2 JV152 LDS1C LDS3 MAD (mothers against decapentaplegic Drosophila) homolog 3 MAD homolog 3 Mad homolog JV15 2 Mad protein homolog MAD mothers against decapentaplegic homolog 3 Mad3 MADH 3 MADH3 MGC60396 Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3 SMA and MAD related protein 3 SMAD 3 SMAD SMAD family member 3 SMAD mothers against DPP homolog 3 Smad3 SMAD3_HUMAN |
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P84022 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
