ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.ICC/IF
Immunofluorescence analysis of A549 cells using FUT1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-FUT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FUT1 |
|---|---|
| Gene Synonyms | FTA Futa H HH HSC MFUT-1 |
| Gene Full Name | fucosyltransferase 1 (H blood group) |
| Gene Summary | This gene encodes a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the synthesis of soluble A and B antigens. This is one of two genes encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group. [provided by RefSeq, Aug 2016] |
| Alternative Names | a-1 2 fucosyltransferase (H antigen) alpha 1 2-fucosyltransferase A alpha 12-fucosyltransferase alpha(1 2) fucosyltransferase 1 alpha(1 2)FT 1 alpha12-fucosyltransferase a beta-galactoside alpha-1 2-fucosyltransferase blood group H alpha 2-fucosyltransferase fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase) galactoside 2-alpha-L-fucosyltransferase galactoside 2-alpha-L-fucosyltransferase 1 galactoside 2-L-fucosyltransferase enzyme GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1 H antigen H glycosyltransferase H glycosytransferase H transferase nonfunctional fucosyltransferase 1 type 1 galactoside alpha-(1 2)-fucosyltransferase FUT1 type 2 galactoside alpha-(1 2)-fucosyltransferase FUT1 |
| Molecular Weight(MW) | 41kDa |
| Source | Rabbit |
| Cellular Localization | Golgi apparatus, Golgi stack membrane |
| SwissProt ID | P19526 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
ICC/IF
Immunofluorescence analysis of A549 cells using FUT1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
