WB
Western blot analysis using ASAP2 antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),THP-1(5),A549(6),Nih3t3(7),Jurkat(8),C2C12(9),Huvec(10),A431(11)cell.WB
Western blot analysis using ASAP2 antibody against Mouse liver tissue(1),Mouse stomach tissue(2),Rat kidney tissue(3),Rat brain tissue(4).IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using ASAP2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human muscle tissues using ASAP2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using ASAP2 antibody with DAB staining.| Product Name | Anti-ASAP2 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ASAP2 |
|---|---|
| Gene Synonyms | 6530401G17Rik AMAP2 CENTB3 DDEF2 Gm1523 Gm592 PAG3 PAP Pap-alpha SHAG1 |
| Gene Full Name | ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 |
| Gene Summary | This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
| Alternative Names | centaurin beta 3 development and differentiation enhancing factor 2 development and differentiation-enhancing factor 2 KIAA0400 PAPbeta paxillin-associated protein with ARF GAP activity 3 PYK2 C terminus-associated protein pyk2 C-terminus-associated protein |
| Molecular Weight(MW) | 111kDa（observed：90kDa) |
| Source | Rabbit |
| Cellular Localization | Cytoplasm;Golgi apparatus, Golgi stack membrane;Cell membrane |
| SwissProt ID | O43150 |
|---|
WB
Western blot analysis using ASAP2 antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),THP-1(5),A549(6),Nih3t3(7),Jurkat(8),C2C12(9),Huvec(10),A431(11)cell.
WB
Western blot analysis using ASAP2 antibody against Mouse liver tissue(1),Mouse stomach tissue(2),Rat kidney tissue(3),Rat brain tissue(4).
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using ASAP2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human muscle tissues using ASAP2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using ASAP2 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
